|Laser||Ex. (nm)||Em. (nm)||Fluorochromes|
|Blue||488||510/23||GFP, FITC, Alexa fluor 488, calcein|
|Yellow-green||561||615/24||dsRed, mCherry, propidium iodide (PI)|
The Biosorter (Union Biometrica) is a jet-in-air sorter that can be used for separating large particles. Unlike FACSJazz and MoFlo Astrios which continuously generate droplets, the Biosorter relies on the air deflection of a continuous liquid stream into a tray.
It can be set up with two FOCAs (Fluidics and Optics Core Assemblies): 250 μm and 1,000 μm and it is equipped with two lasers and three fluorescence detectors.
The Biosorter can be used for purifying or enriching, large cells (megakaryocytes, adipocytes), cell clusters (pancreatic islets, Drosophila imaginal discs), whole organisms (C. elegans, zebrafish larvae) and various other particles (e.g. beads).
|Types of samples that can be sorted on the Biosorter||250 μm FOCA||1,000 μm FOCA|
|Large cells: adipocytes, hepatocytes, megakaryocytes, duct cells||Yes|
|Small cell clusters: stem cell spheres, mammospheres||Yes|
|Small organisms: C. elegans (embryo to adult), Drosophila (embryo to 1st instar)||Yes|
|Beads and other particles (20 - 150 μm)||Yes|
|Larger cell clusters: neurospheres, pancreatic islets, Drosophila imaginal discs.||Yes|
|Plants: Arabidopsis, tobacco (seeds, early seedlings)||Yes|
|Larger organisms: Drosophila (1st to 2nd instar), zebrafish larvae||Yes|
|Beads and other particles with diameter below 700 μm.||Yes|
We encourage end-users to learn how to operate the Biosorter and run their-own sorts. Typically one session of approximately 2 hours is sufficient for gaining basic sorting skills on Biosorter.
Researchers who prefer to have their samples sorted by the staff should contact us to discuss the project in detail.