Biosorter

Status: Ok
Biosorter Laser Ex. (nm) Em. (nm) Fluorochromes
Blue 488     510/23 GFP, FITC, Alexa fluor 488, calcein
Blue 488     543/22 YFP
Yellow-green 561     615/24 dsRed, mCherry, propidium iodide (PI)

The Biosorter (Union Biometrica) is a jet-in-air sorter that can be used for separating large particles. Unlike FACSJazz and MoFlo Astrios which continuously generate droplets, the Biosorter relies on the air deflection of a continuous liquid stream into a tray.

It can be set up with two FOCAs (Fluidics and Optics Core Assemblies): 250 μm and 1,000 μm and it is equipped with two lasers and three fluorescence detectors.

The Biosorter can be used for purifying or enriching, large cells (megakaryocytes, adipocytes), cell clusters (pancreatic islets, Drosophila imaginal discs), whole organisms (C. elegans, zebrafish larvae) and various other particles (e.g. beads).

Types of samples that can be sorted on the Biosorter 250 μm FOCA 1,000 μm FOCA
Large cells: adipocytes, hepatocytes, megakaryocytes, duct cells Yes  
Small cell clusters: stem cell spheres, mammospheres Yes  
Small organisms: C. elegans (embryo to adult), Drosophila (embryo to 1st instar) Yes  
Beads and other particles (20 - 150 μm) Yes  
Larger cell clusters: neurospheres, pancreatic islets, Drosophila imaginal discs.   Yes
Plants: Arabidopsis, tobacco (seeds, early seedlings)   Yes
Larger organisms: Drosophila (1st to 2nd instar), zebrafish larvae   Yes
Beads and other particles with diameter below 700 μm.   Yes

We encourage end-users to learn how to operate the Biosorter and run their-own sorts. Typically one session of approximately 2 hours is sufficient for gaining basic sorting skills on Biosorter.
 

Researchers who prefer to have their samples sorted by the staff should contact us to discuss the project in detail.