CytoFLEX LX

CytoFLEX LX is the newest cytometer in our lab. It has 6 lasers and 21 color detectors and the capability to detect microparticles as small as 200 nm by triggering off the forward scatter detector. Instead of photomultipliers (PMTs) which are commonly used as detectors in most standard flow cytometers, CytoFlex relies on avalanche photodiode (APD) detectors which are characterized by excellent sensitivity and linearity. The characteristics of APDs make the compensation workflow very efficient and CytExpert software used on CytoFlex is easy to learn and operate.
The Cytoflex accommodates 12x75mm tubes, microtiter tubes and has a plate loader module that allows for automatic loading of samples from 96 or 384 well plates.


Laser Ex. (nm)     Em. (nm) Fluorochromes
NUV 375   NUV405 405/30 BUV395
NUV 375   NUV450 450/50 DAPI, Hoechst blue, Live/Dead blue, Zombie UV
NUV 375   NUV675 675/30 BUV661, Hoechst red
Violet 405   V450 450/45 Pacific Blue, BV421, Horizon V450, eFluor 450
Violet 405   V525 525/40 Live/Dead Aqua, BV510, AmCyan, Qdot 525, V500, Krome Orange
Violet 405   V610 610/20 BV605, Qdot 605, eFluor 605
Violet 405   V660 660/10 BV650, Qdot 655, eFluor 650
Violet 405   V763 763/43 BV780
Blue 488   B525 525/40 FITC, Alexa Fluor 488, GFP, CFSE, Fluo-4
Blue 488   B610 610/20 MitoSOX red, PE-CF594, PE-Texas red, PI
Blue 488   B695 690/50 PerCP, PerCP-Cy5.5
Yellow-Green 561   Y585 585/42 PE, dTomato, mOrange
Yellow-Green 561   Y610 610/20 mCherry, PE-CF594, PE-Texas Red, PI
Yellow-Green 561   Y675 675/30 PE-Cy5, 7AAD, PE-AF647, mPlum, PC5
Yellow-Green 561   Y710 710/50 PE-Cy5.5, PE-AF680, PC5.5
Yellow-Green 561   Y763 763/43 PE-Cy7, PC7
Red 638   R660 660/10 APC, Alexa Fluor 647, DRAQ5
Red 638   R712 712/25 Alexa Fluor 700
Red 638   R763 763/43 APC-Cy7, APC-H7, APC-Fire750, APC-eFluor 780, DRAQ7
Infrared 808   IR840 840/20 Alexa fluor 790, IRDye 800CW
Infrared 808   IR885 885/40 PF-840