LSR Fortessa

Status: Ok
Laser Ex. (nm)     Em. (nm) Fluorochromes
UV 355     379/28 BUV395
UV 355     450/50 DAPI, Live/Dead blue, Zombie UV
Violet 405     450/50 Pacific Blue, BV421, Horizon V450, eFluor 450
Violet 405     525/50 Live/Dead Aqua, BV510, AmCyan, Qdot 525
Violet 405     610/20 BV605, Qdot 605, eFluor 605
Violet 405     655/8 BV650, Qdot 655, eFluor 650
Violet 405     710/50 BV711, Qdot 705
Violet 405     780/60 BV780
Blue 488     525/50 FITC, Alexa Fluor 488, GFP, CFSE, Fluo-4
Blue 488     695/40 PerCP, PerCP-Cy5.5
Yellow-Green 561     582/15 PE, dTomato, mOrange
Yellow-Green 561     610/20 mCherry, PE-CF594, PE-Texas Red, PI
Yellow-Green 561     660/20 PE-Cy5, 7AAD, PE-AF647, mPlum
Yellow-Green 561     710/50 PE-Cy5.5
Yellow-Green 561     780/60 PE-Cy7
Red 640     660/20 APC, Alexa Fluor 647, DRAQ5
Red 640     710/50 Alexa Fluor 700
Red 640     780/80 APC-Cy7, APC-H7, APC-Fire750, DRAQ7

LSRFortessa is the most advanced cytometer in the lab. It is equipped with 5 lasers and 18 color detectors. It is operated using the FACSDiva software. In addition to the standard configuration shown above, LSRFortessa can be easily reconfigured to detect YFP and GFP in the same sample.

LSRFortessa links: