LSR II

Status: Ok
LSR II Laser Ex. (nm)     Em. (nm) Fluorochromes
UV 355     379/28 BUV395
UV 355     450/50 DAPI, Live/Dead blue, Zombie UV
Violet 405     450/50 Pacific Blue, BV421, Horizon V450, eFluor 450
Violet 405     525/50 Live/Dead Aqua, Zombie Aqua, BV510, AmCyan, Qdot 525
Violet 405     610/20 BV605, Qdot 605, eFluor605
Violet 405     660/20 BV650, Qdot 655, eFluor 650
Violet 405     710/50 BV711, Qdot 705
Violet 405     780/60 BV785
Blue 488     530/30 FITC, Alexa Fluor 488, GFP, CFSE, Fluo-4
Blue 488     575/26 PE, PI
Blue 488     695/40 PerCP, PerCP-Cy5.5
Blue 488     780/60 PE-Cy7
Red 640     670/30 APC, Alexa Fluor 647, DRAQ5
Red 640     720/40 Alexa Fluor 700
Red 640     780/80 APC-Cy7, APC-H7, APC-Fire750, DRAQ7, L/D-Near IR

LSR II has been recently upgraded with a violet lasers with 6 detectors and an additional detector off the red laser for Alexa fluor 700. LSR II has now 15 color detectors. LSR II and LSRFortessa are operated using the same software, FACSDiva.

LSR links: