MoFlo Astrios EQ

Status: Ok
Laser Ex. (nm)     Em. (nm) Fluorochromes
Violet 405     450/50 Pacific Blue, BV421
Violet 405     525/50 Live/Dead Aqua, BV510, AmCyan, Qdot 525
Violet 405     655/8 BV650, Qdot 655, eFluor 650
Violet 405     710/50 BV711, Qdot 705
Blue 488     513/26 FITC, Alexa Fluor 488, GFP, CFSE, Fluo-4
Blue 488     540/20 YFP
Blue 488     692/75 PerCP-Cy5.5
Yellow-Green 561     582/15 PE, dTomato, mOrange, RFP
Yellow-Green 561     610/20 mCherry, PE-CF594, PE-Texas Red, PI
Yellow-Green 561     660/20 PE-Cy5, 7AAD, mPlum
Yellow-Green 561     780/60 PE-Cy7
Red 640     671/30 APC, Alexa Fluor 647, DRAQ5
Red 640     722/44 Alexa Fluor 700
Red 640     795/70 APC-Cy7, APC-H7, APC-Fire750, DRAQ7

MoFlo Astrios is a high speed cell sorter that can be outfitted with different nozzle sizes (70 and 100 μm are typical). With 4 lasers, 14 color detectors, and ability to detect particles as small as 200 nm using the forward scatter detector, Astrios is the most performant sorter in our lab. Maximum sorting speed with the 70 μm nozzle is 25,000 events/second (approx. 90 million events/hour); with 100 μm nozzle the maximum sorting speed is 10,000 events/second (or 36 million events/hour). Astrios is placed inside a class II biosafety cabinet and it can be used with samples that require containment up to BSL-2 .

Online reservations must be done at least one day in advance and all reservations need to be approved by the staff in order to become effective.

We encourage all end-users to learn how to monitor the sorts and operate the sorter in semi-assisted mode : the instrument is prepared by the staff (laser alignment, laser delays, drop delay etc.) and end users are responsible for feeding the sample and collection tubes into the sorter and monitoring the sort. The staff will be available to troubleshoot incidents and unexpected events.

Researchers interested to learn how to operate MoFlo Astrios independently should contact the staff. At least two training sessions of approximately 2 hours are at minimum required for acquiring the basic knowledge needed for starting up the sorter and setting up a cell sorting experiment. Additional hands-on practice is necessary for operating the sorter in a truly unassisted mode.