Flow cytometry assays

Standard flow cytometry assays

  • Leukocyte immunophenotyping of human blood samples for clinical research studies.
  • Custom multi-color immunophenotyping of leukocyte subpopulations (T, B, NK cells, monocytes)
    • CD4 / CD8 T cell counts for HIV studies
    • T cells subsets (naïve, memory, Treg etc.)
    • NK cells subpopulations and expression of inhibitory / activating receptors
    • Monocytes subpopulations
  • Circulating stem cells and progenitors
  • Intracellular cytokine assays

We routinely run leukocyte immunophenotyping of human blood samples for clinical research studies.

Functional flow cytometry and bead-based assays

  • Apoptosis
  • Cell proliferation and viability
  • Cell cycle
  • Mitochondria membrane potential and generation of reactive oxygen species; mitochondrial mass
  • Intracellular calcium assays by flow cytometry
  • Phagocytosis
  • Detection of intracellular proteins (including cytokines and phosphorylated proteins using phospho-specific antibodies)
  • Cytometric Bead Arrays (CBA) for standard cytokines (Th1, Th2, Th17) or Flex CBA for human or mouse cytokines
  • Natural killer cells cytotoxicity assay